[摘要] 目的 观察硝基酪氨酸在2型糖尿病模型大鼠肾脏的表达及雷米普利(Ramipril)对它的影响。方法 将大鼠分为正常对照组(N)、糖尿病组(D)、雷米普利治疗组(R)，比较各组大鼠肾组织中丙二醛(MDA)含量、超氧化物岐化酶(SOD)活性，和硝基酪氨酸(NT)含量，同时观察血糖、血胰岛素、肾功能和肾脏形态变化。结果 与正常组比较，糖尿病组肾组织中MDA含量明显上升(P 2.2.3 肾组织免疫学检查 常规脱蜡，封闭，一抗37℃孵育2小时，4℃冰箱过夜，标记二抗，作免疫染色，应用高清晰度彩色免疫图像分析系统(IPP55.0)分析，测定肾小球平均光密度直径。
2.2.4 肾脏组织电镜观察 肾组织固定，脱水，包埋，切片，染色，在Hitachi-7500透射电镜下观察。
2.3 统计学方法 实验数据以x±S表示，应用SPSS14统计软件进行处理，两组间数据采用ANOVA检验，若方差不齐，采用非参数统计检验，P0.05)，但其肾重及肾重/体重均明显低于D组(P [3] Daisuke Koya,Kazuyuki Hayashi,Munehiro Kitada,Atsunori Kashiwagi,Ryuichi Kikkawa,and Masakazu Haneda.Effects of Antioxidants in Diabetes-Induced Oxidative Stress in the Glomeruli of Diabetic Rats[J].J.Am.Soc.Nephrol,Aug2003,14:250.
[4] Wu LL,chiou CC,Chang PY,et al.Urinary8-OHdG:a marker of oxidafives tress to DNA and a risk factor for cancer,at heroseclerosis and diabetics[J].Clin Chim Acta,2004,339:1-9.
[5] Nishikawa T,Edelstein D,Du XL,et al.Normalizing mitochomdrial superoxide production blocks three diffrernt pathways of hyperglycemic damage[J].Nature,2000,404:787-790.
[6] Echtay KS,Roussel D,St-Pierre L,et al.Superoxide activates mitochondrialuncouplingp roteins[J].Nature,2002,415:96-99.
[7] Chang JM,Kuo MC,Kuo HT,et al.Increased gomerular and extracellular malondialdehyde levels in patients and rats with diabetic nephropathy[J].J Lab Clin Med,2005,146:210-215.
[8] Ischiropoulos H,Zhu L,Chen J,et al.Peroxynitritemediated tyrosine nitration catalyzed by superoxide dismutase[J].Arch Biochem Briphys,2001,298:431-437.
[9] Qiuling Fan,Jie Liao,et al.Candesartan reduced advanced glycation end-products accumulation and diminished nitro-oxidative stress in type 2 diabetic KK/Ta mice[J].Nephrology Dialysis Transplantation,2004,19(12):3012-3020.
[10] Praveen N.Chander.Nephropathy in Zucker Diabetic Fat Rat Is Associated with Oxidative and Nitrosative Stress:Prevention by Chronic Therapy with a Peroxynitrite Scavenger Ebselen[J].J Am Soc Nephrol,2004,(15):2391-2403.
[11] Yoshida M,Kimura H,Kyuki K,Ito M.Combined effect of probucol and insulin on renal damage in diabetic rats fed a high cholesterol diet[J].Eur J Pharmacol,2006Oct,24,548(1-3):174-80.
[12] Kim DS,Kim HR.Protective effect of calceolarioside on adriamycin-induced cardiomyocyte toxicity[J].Eur J Pharmacol,2006Jul,10,541(1-2):24-32.
[13] Kyoko Umeji, Seiji,Umemoto.Comparative effects of pitavastatin and probucol on oxidative stress,Cu/Zn superoxide dismutase,PPAR-γ,and aortic stiffness in hypercholesterolemia[J].Am J Physiol Heart Circ Physiol,2006,291:H2522-H2532.